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Gravity Perception in Plants: From cell sensing to the biomechanical response – GRAP2
The goal of the project is to study in an interdisciplinary approach involving physicists and biologists the plant response to gravity. A first proposed approach concerns macroscopic experiments at the plant scale: the goal is to obtain precise and systematic measurement of the response of plants to
Membrane dynamics and attachments during gamete interaction – DYNAGAME
Infertility is a major challenge in all occidental and developing countries since 16 % of genitally active couples consult at least once for an infertility problem, and 10% of them will use an assisted reproductive program to achieve their child project. Sperm-oocyte fusion is a major biological eve
Biological functionalisation of silicon substituted hydroxyapatite porous ceramics for bone tissue engineering – Céram-Os
The overall objective of Céram-Os project is to elaborate ceramic scaffolds made of silicon substituted hydroxyapatite functionalized with a peptide sequence of rh-BMP2 (residues 73-92) in order to improve the implants bioactivity, i.e. stimulate both osteogenesis and angiogenesis. Our strategy is
Stabilizing detergents through a salt-bridges network for studying and crystallizing in solution membrane proteins in a functional form. Application to multidrug ABC efflux pumps. – CLAMP
Detergents are key components in the field of structural biology and biochemistry of membranes proteins. They are required for maintaining them in solution for crystallography, ligand screening, antibody production, immunization and other applications. Unfortunately, they tend to unfold these protei
Multifunctional implantable probes based on conducting polymer devices – PolyProbe
PolyProbe is submitted as the continuation of the project MUSIC (Multisensing polymer transistors for in vivo recording), in which we developed implantable microarrays capable of simultaneous recording of electrophysiology and metabolism. MUSIC has been particularly successful. Among its achievement
Theranostic of atherosclerosis using human antibody-targeted multi-modal nanoparticles for in situ delivery of drugs. – ATHERANOS
ATHERANOS project aims at developing a theranostic approach for atherosclerosis which is the main cause of death in the Western world and tends to increase rapidly in developing countries. Atherosclerosis can be regarded as an inflammatory disease that results from an initial activation of the endot
Desmin involvement in mechanical properties of skeletal muscle cell – DESMECA
The cytoskeleton is composed of three networks (microtubules, microfilaments and intermediate filaments), which interact directly or indirectly via associated proteins and plays a central role in the generation and transmission of mechanical forces through the cell. In muscle, it is well established
Oxidation of the amyloid beta peptide and implications in the etiology of Alzheimer's Disease. – AlzABox
The global aim of the project AlzABox is to elucidate, at the molecular level, the role of metal-induced reactive oxygen species (ROS) in the oxidation and aggregation of the amyloid-ß peptide (AB), in relation with Alzheimer’s disease (AD). More specifically, we want to understand the molecular mec
Hypersecretion in severe airway diseases: an integrated biophysical approach of the Mucociliary aspect of the bronchial epithelium. – mucocil
Chronic airway diseases (severe asthma, COPD) concern hundred million of people. Current treatments do not interfere with the natural history of the diseases and do not stop fast decline of lung function which is a hallmark of severity. Morphological and functional abnormalities of the bronchial epi
Neuronal mechanisms of a complex behavior in Aplysia revealed by combined manganese enhanced magnetic resonance imaging and electrophysiology – ANImE
The main goals of the project are: - Develop the MR techniques necessary performing functional MR with single cell resolution. - Demonstrate single cell functional MRI is possible. - Analyze the functional interactions of different neuronal networks in the spontaneous genesis of a complex behav
Study of the N-Terminus Domain of the Androgen Receptor, related to the prostate cancer. – ARIA
Prostate cancer is a leading cause of death of men in the industrialized world. The prostate gland is under a strict hormonal androgen control, and prostate cancer develops initially as an androgen-dependent disease that relies on the androgen receptor (AR) for growth and progression. Relapsed prost
Single-molecule reconstruction of transcription-coupled DNA repair pathways. – RepOne
This DNA repair pathway is essential for repair of bulky DNA lesions generated for instance by UV radiation from sunlight or chemically carcinogenetic reagents contained in tobacco smoke. Understanding this pahtway is essential to the development of novel pharmacological and chemotherapeutic treatm
Dynamics and mechanics of integrins and regulators organization and interactions at the nanoscale in adhesion sites. – Integractome
Cell adhesion and force transmission to and from the ECM are involved in critical cellular functions such as migration,proliferation and differentiation, and their deregulationcontributes to pathologies including cancer. AS are the converging zones integrating biochemical and biomechanical signallin
ANalysis of transcription-induced chromosome DYnamics – ANDY
The objectives of our project are to address the following questions based on the development of innovate technological and biophysical tools: - Do gene relocalization events involve global or local chromosome reorganizations? - How do chromosome structural properties contribute to or evolve dur
Development of a lanthanide-based luminescent nanoparticule for the dynamic quantification of autophagy in vitro and in vivo. – Lumiphage
Self-digestion of the cell, also coined autophagy, is a major biological process involved in the homeostasy of cells and organisms. Its importance is largely recognized in multiple reseach fields of biology such as development, neurodegeneration, cancer, pathogens infection or obesity. As the the ro
Tumor and environment : the role of mechanical stress in cancer invasion – Invaders
The INVADERS project aims to determine the effect of a Mechanical Stress on Cancer Invasion. The current hypotheses favor either bi-directional biochemical signals between the tumor and the surrounding stroma or an integrated biomechanical competition between tumor and stroma. During the project
Activation of the G protein-coupled glutamate receptors : a single molecule study. – NanoGluR
The G protein-coupled receptor (GPCR) family represents more than 30% of all therapeutic drug targets and hence pharmaceutical companies are developing programs to identify new modulators of these receptors. The metabotropic glutamate receptors (mGluRs), are GPCRs activated by glutamate, the major e
Viral Assembly and Membrane Domain Dynamics – FLUOBUDS
Retroviruses are enveloped RNA viruses responsible for many human diseases. Assembly of retroviruses mainly occurs at the cell surface upon the oligomerization of a structural viral protein, namely Gag. Gag anchors the cellular membrane via its N-terminus, namely Matrix (MA), which is myristoylated
Universally high throughput Mass spectrometry-Tag Quantification in biological matrices: Alternative to radiolabeling – MTaQ
We aim at developing a new and universal analytical technology involving non-radioactive detection to quantify a compound of interest in pharmacology experiments such as in competitive binding assays. Indeed, measuring the interaction between a receptor (protein, enzyme, …) and its reference ligand