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Thermal modulation and fluorescence/Raman reading-out for kinetically analyzing networks of chemical/biological reactions – T-KiNet
A COMPLETER A COMPLETER A COMPLETER A COMPLETER We want to introduce a versatile method to characterize mechanisms, and measure the associated rate constants, from analyzing the response of a reactive system to thermal excitations. Our strategy will be applied to networks of reactions, in which the
In vivo biological evaluation of targeted polysaccharide-based NANOcarriers for LUng Cancer treatment – NANOLUC
Non-small cell lung cancer is a very common type of cancer that is currently treated by chemotherapy or radiotherapy with known side effects. Molecularly targeted treatments are now coming to the fore, and epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors have been proposed. However
Organic micro-resonators as label-free chemio- and bio-sensors in micro-fluidic environment – MIRODE
The objective is to develop a high-performance, low fabrication cost sensor, liable to detect very small quantities of chemical (pollutants) or biological (proteins, DNA) species. Biology imposes experimental constraints that have not been integrated in the technologies available in other domains.
211At/64Cu labeled bivalent hapten in a two steps targetting approach (Affinity Enhancement System) – aRIT/ßPET
The use of a radiolabeled protein for targeting cancer cells runs into many pitfalls as unsuitable accumulation time or diffusion to the tumoral cell, instable bonding between the radionuclide and its vector or production process inadequate for human applications. Partner 1 (INSERM U 892) has previ
Endoscopic Mueller Imaging – IMULE
Since the 1990s, optical techniques for medical diagnosis have seen considerable interest, motivated by their non-destructive, minimally invasive and non-contact features. Among them, those based on polarimetry can show contrasts imperceptible with other means, and thus they can reveal potentially c
Mechanically-Induced Morphogenetic Effects – MIME
In Drosophila embryos, mesoderm invagination is generated by mesoderm cells apical constrictions ensured by Fog dependent apical coalescence of Myo-II. This movement is preceded by unstable puslations of apex cells, associated to unstavle apical coalescence of Myo-II. We have suggested that the mech
High-Field Electron Paramagnetic Resonance Spectroscopy of Whole Organisms – SeediEPR
Manganese plays an important role in organisms, including the production of earth’s oxygen supply and also the protection of organisms against the dangerous effects of reactive species formed from oxygen. The chemical distribution of manganese in organisms is poorly understood. The primary goal of
Microbubbles Engineering for Drug Delivery-assisted by Ultrasound – MEDDU
Despite tremendous efforts to develop drug delivery systems, therapeutic index and selectivity toward diseased tissue remain very low. The lack of selectivity often results in high toxicity. The use of ultrasounds coupled to the gaseous microbubbles is promising approach to deliver and reach with n
Nano-sensors for metabolic studies at the single mitochondrion level – NANOMITO
Our fundamental research project positions upstream from biomedical research on mitochondrial pathologies. These pathologies include myopathies, neuropathies and cancers due to deficiencies of a major cell sub-unit: mitochondria. Mitochondria are named the cell powerhouses because they transform bre
Mechanism of inhibition of a novel target of beta-lactam antibiotics – CarbaTub
Tuberculosis is a re-emerging infectious disease caused by the bacterium Mycobacterium tuberculosis. Usually treated by the association of rifampin, isoniazide, pirazinamide, and ethambutol for six months, problems resulting from poor adherence to therapy by the patients in developing countries have
FRET biosensors for spatio-temporal dynamic kinase activity by fast FLIM and fluorescence anisotropy microscopy – KinBioFRET
KinBioFRET proposal is based on an original combination of multidisciplinary partners: technological and methodological development team directly concerned by the development of FRET biosensor methodologies and three biological partners directly interested into dynamics of kinase activities in diffe
Nano-reinforced hydrogel for cartilage regenerative medicine and tissue engineering – HYCAR
We have, since 2006, worked on the development of innovative strategies for regeneration of articular cartilage to reduce the risk of osteoarthritis after a joint focal lesion. To achieve this objective work on the use of chondrocytes from the nasal septum helped prove the concept by combining in an
Mathematical and Physical Modeling of Regenerative Repair – REGENR
The main objective of the project is to do study regenerative tissue repair experimentally and to construct a mathematical model for it. The main animal model will be Zebrafish (Danio Rerio) but the study will also include work on Drosophila (for part of the participants). The study will particular
Characterization of a radical DNA repair enzyme: the spore photoproduct lyase – SPOREPAIR
Bacterial spores are dormant forms adopted by some bacteria under stress conditions. Specific features of spores include a high degree of dehydration, the compaction of the genome in a core protected by walls and membranes, and the absence of metabolic activity. As the result of these specificities,
Controlling photobleaching of fluorescent proteins for super-resolution microscopy. – NOBLEACH
Fluorescence microscopy plays a growing role in life sciences. Recently, super-resolution microscopy (nanoscopy) has been developed, which provides fluorescence images of live samples with ~20 nm spatial resolution, thus opening a huge research field. Nanoscopy will undoubtedly impact the understand
Active and passive mechanics of the mechanosensory hair-cell bundle along the tonotopic axis of the mammalian cochlea. – EARMEC
Hearing starts with the deflection of the hair bundle, an organelle that operates as a mechanical antenna at the surface of each auditory hair cell of the cohlea. Our main objective is to identify the mechanical and molecular mechanism that sets a hair bundle's characteristic frequency of optimal se
Interactions between chromatin and the transcriptional co-activators TFIID, ATAC and SAGA – ChromAct
the level of gene expression, also called transcription, controls normal cell life but also its dysfunctions that promote cancer development or some rare genetic diseases. If the transcription of a particular gene could be controlled the cell fate would be mastered. This project aims at generating n
3D polyelectrolyte scaffolds for prostatic cell culture : a tool to study the influence of the microenvironment on cancerous phenotype and to identify new biomarkers – BROCOLI
The objective of this project is to develop a 3D-microculture format that provides a ductal scaffold of polyelectrolyte microtubes to enable prostatic cells to grow into a normal glandular acinar structure. 3D culture systems for prostatic cells currently utilise a matrix of Matrigel or collagen to
Auxin-dependent dynamics of cell wall xyloglucans – AuxiWall
Our project focuses on the cell wall, and more specifically on the hormonal control of the xyloglucan quality. The acquired data will lead to a better undestanding of the regulation of cell expansion in plants. We anticipate that the knowledge provided within this project will also provide valuable
Single-molecule microscopy of blocked replication forks – MicroRep
The reaction that copies chromosomes (replication) and DNA damage repair are universal processes. In bacteria, as in other organisms, proteins involved in replication and in DNA repair can be observed directly if they are fused to a fluorescent protein. It is known that replication arrest is a sour
Development of new antibacterial and antiparasitic drugs target of the GcpE metalloenzyme – ANTIBIO-T
The emergence and spread of antimicrobial resistance is a serious threat. The development of new drugs acting on new targets is timely. Therefore the elucidation of so far unknown biochemical pathways which are indispensable for the survival of pathogenic bacteria could be a solution to this problem
Neolectins : Synthetic lectins with controled valence and specificity for cellular biology and biotechnology – NeoLect
Interactions between proteins and glycans are an important component of multiple biological functions, including cell signaling, cell migration, immune recognition and interaction with pathogens. However, Glycomics has yet to reach the level of its counterparts, Genomics and Proteomics, due to the d
Photoregulation of the purinergic P2X channel activity: resolution of the gating stoichiometry – Light-GatedP2XR
The atomic structure of each member of the superfamily of ligand-gated ion channels has been recently resolved. This outstanding advance thus encourages the development of new chemical tools to explore in details the functioning of these receptors. P2X receptors are activated by extracellular ATP an
Preclinical development of a method to image brain oxygenation using MRI – IMOXY
Imaging cerebral oxygenation is a key tool for understanding brain function, pathophysiological mechanisms, and to monitor and optimize therapies. Currently, imaging cerebral oxygenation can be achieved in patients using positron emission tomography imaging (PET) technology and near-infrared spectro
photoSRM: optical fragmentation filtering for quantification by mass spectrometry of protein clinical biomarker in biological matrices – photo-SRM
A technological jump is today essential to clear the gap between the up-stream proteomic discovery phases, which usually ends up on the identification of tens of putative biomarkers, and the large clinical validation step based on validated immuno-enzymatic tests. Absolute quantification of proteins
Combining experimental and computational methods to study the impact of biomolecular hydration water on protein dynamics: application to intrinsically disordered proteins and solvent-free protein-polymer hybrids – Bieau
Water is the matrix of life on our planet. It plays fundamental and versatile roles in most biological processes on the cellular and the molecular levels, making water the most important of all biological molecules. Hydration water is indispensable for the structure, stability, dynamics and function
In vivo mechanics during tissue morphogenesis – MorFor
We aim at developing, adapting and applying a set of methods to measure mechanical forces in vivo. ongoing project Tissue engineering and understanding of the mechanics during tissue morphogenesis ongoing Mechanical forces are as important as genes and chemical signals for the control of embryolo
Molybdenum Cofactor Chemistry – MC2
The 20 permanent researchers involved in the present project belong to three CNRS or CEA laboratories and represent the main French research groups working on the mechanism of molybdoenzymes. We have collaborated for the last four years within the framework of a previous ANR project (PCV 2006-2009).