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Mechanics and function of immune cells studied at the single molecule level – ImmuPhy
We aimed at evaluating the contribution of mechanics to the spatio-temporal regulation of cell polarity in B cells by using an innovative interdisciplinary approach –which combines single molecule experiments in live cells (single protein tracking) and force measurements. Our approach will ultimatel
Molecular basis of the vectorial transport of sterol – SterTrans
Sterol is a lipid of vital importance whose accurate distribution in cells results from a combination of synthesis and transport mechanisms between organelles. However, the molecular bases of these transport mechanisms remain largely unknown. The aim of our project was to demonstrate that several pr
Polyion complex micelles for siRNA delivery in immature dendritic cells: polymeric vectors for ex vivo gene silencing in arthritis. – DenSicelles
Dendritic cells (DCs) are professional antigen-presenting cells that play a key-role in maintaining the balance between immunity and tolerance. There is a great deal of interest in how DCs might be exploited as a form of immunotherapy to induce immunity or to restore tolerance. The final goal of thi
DNA polymerases and damaged nucleic acids in archaea: Genome maintenance and Biotechnology – ARCHPOL
ARCHPOL intends to examine the spectra of damaged nucleic acids in P. abyssi, under normal and stressful physiological conditions. Identification and quantitative analyses target the genome and the precursors (dNTPs and NTPs). ARCHPOL also aims at catching novel DNA polymerising enzymes inducible by
Multimodal Fluorescence Light Sheet Microscopy (mFLSM) for in toto imaging of embryonic development – MFLSM-intotomorph
An embryo is shaped by a complex sequence of cell processes and morphogenetic events that are highly regulated both in time and space. Many of these mechanisms, such as collective cell migration, tissue invagination, or cell intercalation, are stereotypical and occur in every organisms. Advances in
Structure and dynamics of bacterial biofilms: from single cells to collective phenomena – FILMOTIL
Bacteria are unicellular prokaryotic microorganisms. They are everywhere and are an important part of the terrestrial biomass. As for most living species, community is necessary for their survival: bacteria self-organize collectively in complex architectures adsorbed on interfaces, the so-called bio
Combined NMR and spin labeling approach for the structural and dynamic studies of RNA/protein complexes: Application to 7SK RNA bound to proteins – RNASPIN
RNAs and their complexes (RNP) are involved in many steps of biological processes. The elucidation of mechanisms of interactions between RNAs and their partners is now a major objective in molecular and structural biology. The key to understand the biological functions of such complexes is to invest
Motion estimation and structure tracking in echocardiography using specific images: the UltraSound Tagging. – US-Tagging
While it has been for a long time the first cause of mortality , cardiovascular pathologies are still the reason for more than 40% of deaths in western country. Their early detection is a major concern of public health. On the other hand medical ultrasound is not only the safest and cheapest imaging
Structural basis of molecular recognition in intrinsically disordered proteins – ProteinDisorder
Classical structural biology has experienced a shift towards a more dynamic paradigm with the realization that a protein can be fully functional even in the absence of a stable, folded structure. It is estimated that up to 40% of the proteins encoded by the human genome are intrinsically disordered
Structural, dynamic and functional approach of the Hsp90 chaperone machinery: exploration by conformational probes – MACHsp90
In September 2007 in its weekly epidemiological bulletin the Institut National de Veille Sanitaire (INVS) as announced that for the first time in France, cancer is the leading cause of death before cardiovascular diseases. Indeed, cancer incidence as doubled between 1980 and 2005. Therefore, the dev