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Exploring Global RNA-RNA Interactions in Bacteria – Duplex-Omics
Unlike that of miRNA in eukaryotes, the action of bacterial regulatory RNAs (or small RNAs – sRNAs) is poorly characterized in terms of conservation, recognition and thermodynamics constraints. The set of fully characterized sRNA-target interactions is very small and computer programs predicting RNA
Combining mass spectrometry and functional analyses for a comprehensive study of the phosphorylation of the Cav3.2 T-type calcium channel – Phospho-Cav
L’objectif de Phospho-Cav est l’étude de la phosphorylation des canaux calciques de type T. S’il est aujourd’hui reconnu que ces canaux T sont régulés fonctionnellement par des sérine/thréonine kinases, les modalités de cette régulation sont encore inconnues. Le challenge relevé par le projet Phosph
Replication and instability of common fragile sites – repinsCFS
Several arguments suggested the way DNA is duplicated at these fragile sites during cell division (replication) could present some peculiarities that would explain their fragility. Therefore we have studied in details DNA replication at these sites. We have demonstrated that, contrary to common beli
Mono or poly ovulation, evolutionary role of the BMP15 gene in mammals – MONOPOLY
Among mammals, there is a strong variation in the number of ovulations within the ovaries at each sexual cycle and therefore, the number of newborns at each birth. Indeed, the number of newborns can be 1 or 2 for women, cow or sheep, but more than 10 for rodents or pig. Natural mutations in sheep in
The involvement of chromatin structure in DNA repair – ChromaRep
x x x x The integrity of our genome is constantly challenged by various assaults. DNA damage can occur due to both environmental agents such as UV light or irradiation, and endogenous sources, such as oxidative by-products of cellular metabolism or stalled replication forks. To prevent irreversible
Unravelling Genome Biology through a unique combination of comparative Genomics, functional Genomics and population Genomics (3G) – GB-3G
Basic research in biology has developed around model organisms representing only few of the countless living species. For most of the model organisms, they are evolutionary highly divergent and these large distances do not allow to follow in detail the mechanisms that govern the evolution of basic c
Application of PICh for the characterization of the RNA Polymerase III transcription regulome – PIChClassIII
RNA polymerase III plays a major role in the coordination of growth cell rate due to physiological or environmental changes, especially when cells are exposed to stress conditions. We intend to decipher the pathways (conserved from yeast to human) involved in the regulation of transcription, from th
Impact of the biotrophic transportome on mutualistic plant-fungal interactions. – TRANSMUT
Understanding mechanisms underlying high nutrients use efficiency and carbon allocation in a context of mycorrhizal interactions is critical for sound management of both croplands and forests taking care of ecosystem services rendered by mycorrhizal fungi. Indeed, availability, uptake and exchange o
A genome-wide study of nucleosome positioning in Paramecium tetraurelia nuclei – GENOMAC
In the nuclei of living cells, DNA is wrapped around protein complexes to form the primary units of chromatin that are called nucleosomes. Numerous studies have shown that this packaging is not random and that nucleosome positioning plays an important role, in particular in the control of gene expre
Global analysis of PTC-containing RNA regulation by the NMD pathway and the EJC-like in intron-rich and intron-poor yeasts – YeastIntrons
Introns and their splicing regulation have been mainly studied in metazoans although a lot of technical difficulties are associated with the study of these organisms. We have proposed to study intron splicing in fungi for which very little studies had been published concerning this matter. We had ch
Deciphering beta-arrestin-dependent signalling network engaged by G protein-coupled receptors. – GPCRnet
G protein-coupled receptors (GPCR) are involved in most physiological and pathological processes. Moreover, they are targeted by almost 50% of currently marketed drugs. The goal of GPCRnet is to gain a better understanding of the mechanisms which transmit the information flux from activated GPCRs to
Generating tools for functional genomics in C. elegans – Elegineer
xxx xxx xxx xxx The nematode Caenorhabditis elegans is a widely appreciated, powerful system in which to study fundamental biological mechanisms ultimately related to human health. We propose to develop novel genomic tools to manipulate the genome of C. elegans. First, we plan to generate new resou
Functions of glutaredoxins in Iron and REdox Sensing and signaling in plants – FIRES
Iron containing proteins are essential for many metabolic pathways or cellular processes (photosynthesis or respiration). However, regulation mechanisms of iron homeostasis and the functioning of iron-sulfur cluster assembly machineries in plants are not very well understood. This project aims at un
Spatiotemporal program of replication of the human genome – REFOPOL
Our goal is to identify the location and activation time of each of these sites by determining the local direction of copying on the whole set of chromosomes. The results allow us to understand in each cell type the link between chromosomal organisation in the nucleus, gene expression, temporal orde
From gene regulatory networks to organ shape: an interdisciplinary study of leaf development. – MorphoLeaf
Leaves can show diverse levels of dissections ranging from no (entire margin), mild (serration) to strong (lobes) incisions. The leaf itself can be either simple or dissected into units called leaflets. We have identified an evolutionary-conserved genetic factor the CUC genes that are required for t
Genomewide Genetic Screening to Identify New Regulators of the NF-kB Signaling Pathway – kB-Regs
It is proposed here to identify novel (positive or negative) regulators of the NF-?B pathway. More precisely, we will search for participants in the innate immune response which fights infection, in the DNA damage response triggered by chimiotherapeutic treatments, which is dampened by NF-kB activat
Integrated and functional approach of S-Palmitoylated and N-Myristoylated proteomes in Arabidopsis – PalMyProt
This projects aims at investigating the space and function of two major lipid protein modification of protein N-termini, N-Myristoylation (MYR) and S-palmitoylation (PAL) by integrating two double-level of analysis (i) a general qualitative level of the complete proteome and another level more focus
Regulation of chloroplast proteome dynamics – Chloro-Pro
During the course of a collaborative project (ANR Génoplante 2006; project “GlycoChloroplast”) between the two laboratories (LPCV and EDyP), we produced important tools to investigate the dynamics of the chloroplast proteome at the scale of a full organelle. One important and yet unique tool is the
Formation, dynamics and epigenetic functions of 5-hydroxymethylcytosine residues in DNA. – EPIGENOME
This project aims to study the function of 5-hydroxymethylcytosine (5hmC) residues ??in epigenetic regulations and genetic instability. Our objectives are structured in four tasks: i) Role of TET2 protein, oxidative stress and enzymes involved in DNA repair on the formation and dynamics of the 5hmC
Regulation of plant cell death by the MAPK-IPS1 signalling network – MAPK
Comprendre comment les plantes induisent la mort cellulaire est important pour comprendre qu’est ce qui se passe apres une attaque par des pathogenes mais egalement quand chaque automn des tonnes des feuilles meurent et sont sacrifiees par des plantes vivaces. L’identification des regulateurs majeur