Un module intégré de phénotypage permettant le suivi des réponses immunitaires anti-infectieuses

The program EQUIPEX/PHENOMIX allows us to develop novel services to pioneer multiparametric standardized immunophenotyping of mutant mice under normal and pathological conditions (inflammation, infection, cancer).  The PHENOMIX project assembled an integrated advanced immunophenotyping flow, spectral and mass cytometry suite and a confined BSL2/3 module. These technological facilities allow to challenge the immune system of mice with pathogens/vaccines/tumors and to analyse the corresponding biological samples using advanced flow cytometry and microscopy techniques.

CIPHE is a service unit specifically dedicated 1) to develop innovative knockin-knockout mouse lines in a fast track manner, 2) to develop reporter strains allowing to track and ablate specific populations of cells belonging to the innate and adaptive immune systems, 3) to phenotype in a comprehensive manner the mouse and human immune system with a state-of-the-art polychromatic flow cytometry, spectral and mass cytometry suite allowing to quantify over 200 parameters in a standardized manner, and 4) to phenotype and image in vivo the immune system of mutant mice challenged with both microbial and viral pathogens under BSL2/3 conditions.