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Centre d'analyse de systèmes complexes dans les environnements complexes (CACSICE)


Action : Equipements d'excellence


N° de convention : 11-EQPX-0008

Informations générales

  • Référence projet : 11-EQPX-0008
  • RST : Michael NILGES
  • Etablissement Coordinateur : Institut Pasteur
  • Région du projet : Île-de-France
  • Discipline : 5 - Bio Med
  • Aide allouée : 7 515 770 €
  • Date de début du projet : 01/10/2012
  • Date de fin du projet : 31/12/2019
  • Site web du projet : http://aria.pasteur.fr/cacsice
  • Mots clés : integrative structural biology; cellular structural biology; hybrid methods

Résumé du projet

Cells function through transient interactions of macromolecular complexes. Understanding fully the dynamic processes of life at the molecular level requires understanding the structures of these dynamic complexes. Developing the tools to accomplish this aim is the subject of CACSICE: we are creating a centre dedicated to studying the spatio-temporal complexity of macromolecular assemblies and macromolecules in living systems. Historically, structural biology has been largely confined to studying static structures of a single molecule with a single technique, in most cases X-ray crystallography. To understand the formation and evolution of transient complexes within a living cell, we need a radical change in structural biology approaches. Data acquired with multiple biophysical techniques at multiple scales must be collected and integrated into one consistent, dynamic, picture that relies both on emerging experimental technologies and on modelling and numerical simulations. To put this multi-site platform into operation is in itself a challenging task. The first technologies are in operation, notably a novel mass spectrometer that allows one analyse the large dynamic molecular complexes; also, through the installation of a new direct detection camera, the obtainable resolution of a 200 kV microscope has been dramatically increased. Further major installations are foreseen: a 700 MHz solid / liquid state NMR spectrometer has recently been installed at the IBPC in 2016; and the Titan Krios 300 kV microscope will be installed starting in April 2017 at the Institut Pasteur in a new dedicated building.

(L'auteur de ce résumé est le coordinateur du projet, qui est responsable du contenu de ce résumé. L'ANR décline par conséquent toute responsabilité quant à son contenu.)